Effect of coconut water and monosodium glutamate on chrysanthemum explant growth in vitro

Abstract

This study aims to determine the effect of coconut water and MSG on the growth of chrysanthemum explants in vitro. This study is held in the Tissue Culture Lab of the Faculty of Agriculture of Universitas Klabat from September 2019 to January 2020. This study uses a completely randomized factorial design with two treatment factors, namely coconut water and MSG. The variables observed were the number of shoots, plant heights, and the number of leaves. Observations were made each week for 8 weeks after the explants were planted. Coconut’s water dosage that gives the highest number of shoots is 15%, while the dosage for the tallest plant heights and the most leaves is 30%. The dosage of MSG that gives the best result for the number of shoots is 2 ppm, and 1 ppm for plant heights and the number of leaves. The best combination of coconut water and MSG for the number of shoots is 15% coconut water and 0 ppm MSG; for plant heights, 30% coconut water and 2 ppm MSG; and for the number of leaves, 15% coconut water and 0 ppm MSG.

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